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e coli cft073 wam2267 upec strain  (ATCC)


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    Structured Review

    ATCC e coli cft073 wam2267 upec strain
    Fig. 1 | Experimental and analytical workflow for the investigation of the proteome of the bacter- iophage virome and of urinary tract infection (UTI) pathogens in human urine. Affinity hydro- gel spheres are mixed with urine samples; captured proteins are eluted and processed for discovery mass spectrometry proteomic analysis. Tandem spectra are matched with a protein database of bacter- iophages, Homo sapiens, and of UTI pathogens (uropathogenic <t>Escherichia</t> <t>coli</t> UPEC, Escherichia coli 0157, Escherichia coli O25b, Enterobacter cloa- cae, Enterococcus faecalis, Staphylococcus aureus, Streptococcus agalactiae, Proteus mirabilis, Pseudo- monas aeruginosa, Klebsiella pneumoniae, and Bacteriophages databases) retrieved from the Uni- prot and NCBI repositories. Created in https:// BioRender.com.
    E Coli Cft073 Wam2267 Upec Strain, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 255 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/e+coli+cft073+wam2267+upec+strain/pm39905205-280-18-24?v=ATCC
    Average 96 stars, based on 255 article reviews
    e coli cft073 wam2267 upec strain - by Bioz Stars, 2026-08
    96/100 stars

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    1) Product Images from "Urinary bacteriophage cooperation with bacterial pathogens during human urinary tract infections supports lysogenic phage therapy."

    Article Title: Urinary bacteriophage cooperation with bacterial pathogens during human urinary tract infections supports lysogenic phage therapy.

    Journal: Communications biology

    doi: 10.1038/s42003-025-07598-8

    Fig. 1 | Experimental and analytical workflow for the investigation of the proteome of the bacter- iophage virome and of urinary tract infection (UTI) pathogens in human urine. Affinity hydro- gel spheres are mixed with urine samples; captured proteins are eluted and processed for discovery mass spectrometry proteomic analysis. Tandem spectra are matched with a protein database of bacter- iophages, Homo sapiens, and of UTI pathogens (uropathogenic Escherichia coli UPEC, Escherichia coli 0157, Escherichia coli O25b, Enterobacter cloa- cae, Enterococcus faecalis, Staphylococcus aureus, Streptococcus agalactiae, Proteus mirabilis, Pseudo- monas aeruginosa, Klebsiella pneumoniae, and Bacteriophages databases) retrieved from the Uni- prot and NCBI repositories. Created in https:// BioRender.com.
    Figure Legend Snippet: Fig. 1 | Experimental and analytical workflow for the investigation of the proteome of the bacter- iophage virome and of urinary tract infection (UTI) pathogens in human urine. Affinity hydro- gel spheres are mixed with urine samples; captured proteins are eluted and processed for discovery mass spectrometry proteomic analysis. Tandem spectra are matched with a protein database of bacter- iophages, Homo sapiens, and of UTI pathogens (uropathogenic Escherichia coli UPEC, Escherichia coli 0157, Escherichia coli O25b, Enterobacter cloa- cae, Enterococcus faecalis, Staphylococcus aureus, Streptococcus agalactiae, Proteus mirabilis, Pseudo- monas aeruginosa, Klebsiella pneumoniae, and Bacteriophages databases) retrieved from the Uni- prot and NCBI repositories. Created in https:// BioRender.com.

    Techniques Used: Infection, Mass Spectrometry

    Fig. 2 | Phage protein abundance linearly correlates with bacterial protein abundance but the phage/bacteria ratio is inversely proportional to bacterial abundance. Phages that are annotated to infect uropathogenic bacterial are con- sidered here. A, B The protein abundance of bacteria versus phages in UTI cases and controls are plotted; every dot is a patient. Grey lines depict 1:1 linear relationship. Slope m and R2 values refer to linear regressions; P values are derived from a two- sided t-test against a slope ≠1. Protein abundance of all uropathogenic bacteria considered (uropathogenic Escherichia coli (UPEC), Enterobacter cloacae, Enter- ococcus faecalis, Staphylococcus aureus, Streptococcus agalactiae, Proteus mirabilis, Pseudomonas aeruginosa, Klebsiella pneumoniae) and their phages is reported in (A), protein abundance of E. coli and its phages is reported in (B). C UTI patients had a higher likelihood of temperate phenotype phage protein detection compared to non-UTI controls (odd ratio = 1.47, 95% CI 1.11–2.11, Fisher exact test p = 0.03). The phage phenotype was predicted by BACPHLIP14.
    Figure Legend Snippet: Fig. 2 | Phage protein abundance linearly correlates with bacterial protein abundance but the phage/bacteria ratio is inversely proportional to bacterial abundance. Phages that are annotated to infect uropathogenic bacterial are con- sidered here. A, B The protein abundance of bacteria versus phages in UTI cases and controls are plotted; every dot is a patient. Grey lines depict 1:1 linear relationship. Slope m and R2 values refer to linear regressions; P values are derived from a two- sided t-test against a slope ≠1. Protein abundance of all uropathogenic bacteria considered (uropathogenic Escherichia coli (UPEC), Enterobacter cloacae, Enter- ococcus faecalis, Staphylococcus aureus, Streptococcus agalactiae, Proteus mirabilis, Pseudomonas aeruginosa, Klebsiella pneumoniae) and their phages is reported in (A), protein abundance of E. coli and its phages is reported in (B). C UTI patients had a higher likelihood of temperate phenotype phage protein detection compared to non-UTI controls (odd ratio = 1.47, 95% CI 1.11–2.11, Fisher exact test p = 0.03). The phage phenotype was predicted by BACPHLIP14.

    Techniques Used: Quantitative Proteomics, Bacteria, Derivative Assay



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    e coli cft073 wam2267 upec strain  (ATCC)
    96
    ATCC e coli cft073 wam2267 upec strain
    Fig. 1 | Experimental and analytical workflow for the investigation of the proteome of the bacter- iophage virome and of urinary tract infection (UTI) pathogens in human urine. Affinity hydro- gel spheres are mixed with urine samples; captured proteins are eluted and processed for discovery mass spectrometry proteomic analysis. Tandem spectra are matched with a protein database of bacter- iophages, Homo sapiens, and of UTI pathogens (uropathogenic <t>Escherichia</t> <t>coli</t> UPEC, Escherichia coli 0157, Escherichia coli O25b, Enterobacter cloa- cae, Enterococcus faecalis, Staphylococcus aureus, Streptococcus agalactiae, Proteus mirabilis, Pseudo- monas aeruginosa, Klebsiella pneumoniae, and Bacteriophages databases) retrieved from the Uni- prot and NCBI repositories. Created in https:// BioRender.com.
    E Coli Cft073 Wam2267 Upec Strain, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/e+coli+cft073+wam2267+upec+strain/pm39905205-280-18-24?v=ATCC
    Average 96 stars, based on 1 article reviews
    e coli cft073 wam2267 upec strain - by Bioz Stars, 2026-08
    96/100 stars
    		  Buy from Supplier

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    Fig. 1 | Experimental and analytical workflow for the investigation of the proteome of the bacter- iophage virome and of urinary tract infection (UTI) pathogens in human urine. Affinity hydro- gel spheres are mixed with urine samples; captured proteins are eluted and processed for discovery mass spectrometry proteomic analysis. Tandem spectra are matched with a protein database of bacter- iophages, Homo sapiens, and of UTI pathogens (uropathogenic Escherichia coli UPEC, Escherichia coli 0157, Escherichia coli O25b, Enterobacter cloa- cae, Enterococcus faecalis, Staphylococcus aureus, Streptococcus agalactiae, Proteus mirabilis, Pseudo- monas aeruginosa, Klebsiella pneumoniae, and Bacteriophages databases) retrieved from the Uni- prot and NCBI repositories. Created in https:// BioRender.com.

    Journal: Communications biology

    Article Title: Urinary bacteriophage cooperation with bacterial pathogens during human urinary tract infections supports lysogenic phage therapy.

    doi: 10.1038/s42003-025-07598-8

    Figure Lengend Snippet: Fig. 1 | Experimental and analytical workflow for the investigation of the proteome of the bacter- iophage virome and of urinary tract infection (UTI) pathogens in human urine. Affinity hydro- gel spheres are mixed with urine samples; captured proteins are eluted and processed for discovery mass spectrometry proteomic analysis. Tandem spectra are matched with a protein database of bacter- iophages, Homo sapiens, and of UTI pathogens (uropathogenic Escherichia coli UPEC, Escherichia coli 0157, Escherichia coli O25b, Enterobacter cloa- cae, Enterococcus faecalis, Staphylococcus aureus, Streptococcus agalactiae, Proteus mirabilis, Pseudo- monas aeruginosa, Klebsiella pneumoniae, and Bacteriophages databases) retrieved from the Uni- prot and NCBI repositories. Created in https:// BioRender.com.

    Article Snippet: Determination of prophage induction, excision, integration, and transposition rates E. coliB (AmericanTypeCulture Collection, ATCC catalog number 11303) and E. coli CFT073 [WAM2267] UPEC strain (ATCC catalog number 700928) was spiked in 5mL of urine from UTI and non-UTI human participants diluted 1:1 with LB broth at a concentration of 10^8 cells/mL.

    Techniques: Infection, Mass Spectrometry

    Fig. 2 | Phage protein abundance linearly correlates with bacterial protein abundance but the phage/bacteria ratio is inversely proportional to bacterial abundance. Phages that are annotated to infect uropathogenic bacterial are con- sidered here. A, B The protein abundance of bacteria versus phages in UTI cases and controls are plotted; every dot is a patient. Grey lines depict 1:1 linear relationship. Slope m and R2 values refer to linear regressions; P values are derived from a two- sided t-test against a slope ≠1. Protein abundance of all uropathogenic bacteria considered (uropathogenic Escherichia coli (UPEC), Enterobacter cloacae, Enter- ococcus faecalis, Staphylococcus aureus, Streptococcus agalactiae, Proteus mirabilis, Pseudomonas aeruginosa, Klebsiella pneumoniae) and their phages is reported in (A), protein abundance of E. coli and its phages is reported in (B). C UTI patients had a higher likelihood of temperate phenotype phage protein detection compared to non-UTI controls (odd ratio = 1.47, 95% CI 1.11–2.11, Fisher exact test p = 0.03). The phage phenotype was predicted by BACPHLIP14.

    Journal: Communications biology

    Article Title: Urinary bacteriophage cooperation with bacterial pathogens during human urinary tract infections supports lysogenic phage therapy.

    doi: 10.1038/s42003-025-07598-8

    Figure Lengend Snippet: Fig. 2 | Phage protein abundance linearly correlates with bacterial protein abundance but the phage/bacteria ratio is inversely proportional to bacterial abundance. Phages that are annotated to infect uropathogenic bacterial are con- sidered here. A, B The protein abundance of bacteria versus phages in UTI cases and controls are plotted; every dot is a patient. Grey lines depict 1:1 linear relationship. Slope m and R2 values refer to linear regressions; P values are derived from a two- sided t-test against a slope ≠1. Protein abundance of all uropathogenic bacteria considered (uropathogenic Escherichia coli (UPEC), Enterobacter cloacae, Enter- ococcus faecalis, Staphylococcus aureus, Streptococcus agalactiae, Proteus mirabilis, Pseudomonas aeruginosa, Klebsiella pneumoniae) and their phages is reported in (A), protein abundance of E. coli and its phages is reported in (B). C UTI patients had a higher likelihood of temperate phenotype phage protein detection compared to non-UTI controls (odd ratio = 1.47, 95% CI 1.11–2.11, Fisher exact test p = 0.03). The phage phenotype was predicted by BACPHLIP14.

    Article Snippet: Determination of prophage induction, excision, integration, and transposition rates E. coliB (AmericanTypeCulture Collection, ATCC catalog number 11303) and E. coli CFT073 [WAM2267] UPEC strain (ATCC catalog number 700928) was spiked in 5mL of urine from UTI and non-UTI human participants diluted 1:1 with LB broth at a concentration of 10^8 cells/mL.

    Techniques: Quantitative Proteomics, Bacteria, Derivative Assay